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The Effect of High Intensity Interval Training and Probiotic Consumption on TGF-β1 Gene Expression in Heart Tissue of Male Rats with Steatosis
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Saeed Shahmohammadi1    , Farshad Ghazalian *1 , Hossein Shirvani1 , Hossein Abednatanzi |
| 1- Physical Education Department, Science and Research Branch,Islamic Azad University, Tehran, Iran |
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Abstract: (21 Views) |
Introduction: Given the increasing prevalence of fatty liver disease and its association with other conditions-such as cardiovascular diseases-effective treatment strategies are essential.
Objective: the aim of this study was to evaluate the effect of high-intensity interval training (HIIT) and Lactobacillus rhamnosus supplementation on the expression of the TGF-β1 gene in cardiac tissue of male Wistar rats with steatosis.
Materials and Methods: In this experimental trial, 40 male rats (weighing 200–250 grams) were. assigned to five groups (n=8 per group). To induce steatosis in groups 2 through 5, rats received a daily oral gavage of a high-fat emulsion (10 mg/kg) for six weeks. Additionally, rats in groups 4 and 5 were administered Lactobacillus rhamnosus GG at a concentration of 10⁹ CFU/mL via oral gavage for five weeks, five days per week. Fatty liver induction was confirmed by measuring serum levels of liver enzymes (AST, ALT, and ALP). Following anesthesia and heart tissue extraction, cardiac TGF-β1 gene expression was assessed using real-time PCR.
Results: the expression level of the TGF-β1 gene was significantly elevated in the steatosis group compared to the healthy control group (P=0.001). While all intervention groups showed reduced expression levels of TGF-β1, the most significant decrease was observed in the steatosis + HIIT+probiotic group when compared to the steatosis group alone (P=0.003).
Conclusion: Although five weeks of high-intensity interval training combined with probiotic supplementation significantly reduced TGF-β1 expression in rats, further research is needed before recommending this combined intervention in human populations. |
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| Keywords: Exercise Training, Lactobacillus rhamnosus GG, Liver Disease, Probiotic, Non-Alcoholic Fatty |
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Full-Text [PDF 664 kb]
(23 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2023/08/22 | Revised: 2023/11/11 | Accepted: 2023/11/27 | Published: 2025/03/30
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